› 2pkw

X-ray diffraction
2.1Å resolution

Crystal structure of UPF0341 protein yhiQ from Salmonella typhimurium, Northeast Structural Genomics Consortium Target StR221

Released:
DOI: 10.2210/pdb2pkw/pdb
PDB entry 2pkw coloured by chain, front view.
PDB entry 2pkw coloured by chain, side view.
PDB entry 2pkw coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Entry authors: Forouhar F, Su M, Seetharaman J, Fang Y, Xiao R, Cunningham K, Ma L-C, Owens L, Liu J, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(1516) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1516) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194930 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase J Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 28.78 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X6G2 (Residues: 1-252; Coverage: 100%)
Gene names: STM3593, rsmJ, yhiQ
Sequence domains: Putative SAM-dependent methyltransferase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 41.967Å b: 70.248Å c: 77.788Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.259
Expression system: Escherichia coli

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