› 2pl3

X-ray diffraction
2.15Å resolution

Human DEAD-box RNA helicase DDX10, DEAD domain in complex with ADP

Released:
DOI: 10.2210/pdb2pl3/pdb
PDB entry 2pl3 coloured by chain, front view.
PDB entry 2pl3 coloured by chain, side view.
PDB entry 2pl3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Comparative structural analysis of human DEAD-box RNA helicases.
Schütz P, Karlberg T, van den Berg S, Collins R, Lehtiö L, Högbom M, Holmberg-Schiavone L, Tempel W, Park HW, Hammarström M, Moche M, Thorsell AG, Schüler H
PLoS One 5 (2010)
PMID: 20941364

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable ATP-dependent RNA helicase DDX10 Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13206 (Residues: 47-280; Coverage: 27%)
Gene name: DDX10
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
Ligand EOH 4 x EOH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P6122
Unit cell:
a: 63.5Å b: 63.5Å c: 304.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.21 0.248
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Dbp5, Gle1-IP6 and Nup159: a working model for mRNP export.
Folkmann et al. (2011)
15 more

4 mentions without citation

The application of FAST-NMR for the identification of novel drug discovery targets.
Powers et al. (2008)
3 more