› 2pla

X-ray diffraction
2.51Å resolution

Crystal structure of human glycerol-3-phosphate dehydrogenase 1-like protein

Released:
DOI: 10.2210/pdb2pla/pdb
PDB entry 2pla coloured by chain, front view.
PDB entry 2pla coloured by chain, side view.
PDB entry 2pla coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Uppenberg J, Smee C, Hozjan V, Kavanagh K, Bunkoczi G, Papagrigoriou E, Pike ACW, Ugochukwu E, Umeano C, von Delft F, Weigelt J, Arrowsmith CH, Edwards A, Sundstrom M, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
sn-glycerol 3-phosphate + NAD(+) = glycerone phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185369 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol-3-phosphate dehydrogenase 1-like protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 38.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N335 (Residues: 1-349; Coverage: 99%)
Gene names: GPD1L, KIAA0089
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand PO4 3 x PO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 139.1Å b: 139.1Å c: 109.62Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.175 0.212
Expression system: Escherichia coli BL21(DE3)

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