› 2ply

X-ray diffraction
2.6Å resolution

Structure of the mRNA binding fragment of elongation factor SelB in complex with SECIS RNA.

Released:
DOI: 10.2210/pdb2ply/pdb
PDB entry 2ply coloured by chain, front view.
PDB entry 2ply coloured by chain, side view.
PDB entry 2ply coloured by chain, top view.
Source organism: Moorella thermoacetica
Primary publication:
Structural insight into a molecular switch in tandem winged-helix motifs from elongation factor SelB.
Soler N, Fourmy D, Yoshizawa S
J Mol Biol 370 728-41 (2007)
PMID: 17537456

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-175119 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Selenocysteine-specific elongation factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 29.74 KDa
Source organism: Moorella thermoacetica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46455 (Residues: 377-634; Coverage: 41%)
Gene name: selB
Sequence domains:
Structure domains:
RNA (5'-R(*GP*GP*CP*GP*UP*UP*GP*CP*CP*GP*GP*UP*CP*UP*GP*GP*CP*AP*AP*CP*GP*CP*C)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 23 nucleotides
Theoretical weight: 7.39 KDa
Source organism: Moorella thermoacetica
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand MG 17 x MG
Ligand NA 1 x NA
Ligand CA 2 x CA
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2
Unit cell:
a: 158.64Å b: 120.838Å c: 50.865Å
α: 90° β: 100.18° γ: 90°
R-values:
R R work R free
0.23 0.227 0.287
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

From keys to bulldozers: expanding roles for winged helix domains in nucleic-acid-binding proteins.
Harami et al. (2013)
2 more

1 mention without citation

Delivery of seasonal malaria chemoprevention with enhanced infection prevention and control measures during the COVID-19 pandemic in Nigeria, Burkina Faso and Chad: a cross-sectional study.
Ward et al. (2022)