› 2pn0

X-ray diffraction
1.7Å resolution

Prokaryotic transcription elongation factor GreA/GreB from Nitrosomonas europaea

Released:
DOI: 10.2210/pdb2pn0/pdb
PDB entry 2pn0 coloured by chain, front view.
PDB entry 2pn0 coloured by chain, side view.
PDB entry 2pn0 coloured by chain, top view.
Source organism: Nitrosomonas europaea
Entry authors: Osipiuk J, Evdokimova E, Kagan O, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prokaryotic transcription elongation factor GreA/GreB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 141 amino acids
Theoretical weight: 15.68 KDa
Source organism: Nitrosomonas europaea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82T10 (Residues: 1-137; Coverage: 100%)
Gene names: NE2130, rnk
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 12 x MSE
Ligand OCS 4 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 76.628Å b: 80.838Å c: 171.387Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.206
Expression system: Escherichia coli BL21(DE3)

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