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X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF AN URONATE ISOMERASE (BH0493) FROM BACILLUS HALODURANS C-125 AT 2.00 A RESOLUTION

Released:
DOI: 10.2210/pdb2pnk/pdb
PDB entry 2pnk coloured by chain, front view.
PDB entry 2pnk coloured by chain, side view.
PDB entry 2pnk coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-191862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BH0493 protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 428 amino acids
Theoretical weight: 50.86 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KFI6 (Residues: 1-427; Coverage: 100%)
Gene name: BH0493
Structure domains: uronate isomerase, domain 2, chain A

Ligands and Environments

8 bound ligands:
Ligand NA 3 x NA
Ligand CL 8 x CL
Ligand FMT 14 x FMT
Ligand GOL 24 x GOL
Ligand UNL 3 x UNL
Ligand PO4 4 x PO4
Ligand MPD 6 x MPD
Ligand ACT 3 x ACT
1 modified residue:
Ligand MSE 204 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 273.72Å b: 158.56Å c: 181.24Å
α: 90° β: 116.03° γ: 90°
R-values:
R R work R free
0.149 0.148 0.178
Expression system: Escherichia coli

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