› 2pnw

X-ray diffraction
1.9Å resolution

Crystal structure of membrane-bound lytic murein transglycosylase from Agrobacterium tumefaciens

Released:
DOI: 10.2210/pdb2pnw/pdb
PDB entry 2pnw coloured by chain, front view.
PDB entry 2pnw coloured by chain, side view.
PDB entry 2pnw coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Entry authors: Bonanno JB, Dickey M, Bain KT, Iizuka M, Ozyurt S, Smith D, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
peptidoglycan lytic exotransglycosylase Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 42.32 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A9CKT8 (Residues: 2-370; Coverage: 100%)
Gene names: Atu0009, mltA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P6422
Unit cell:
a: 88.665Å b: 88.665Å c: 221.434Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.219
Expression system: Escherichia coli BL21(DE3)

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