› 2pof

X-ray diffraction
1.4Å resolution

Crystal structure of CDP-diacylglycerol pyrophosphatase

Released:
DOI: 10.2210/pdb2pof/pdb
PDB entry 2pof coloured by chain, front view.
PDB entry 2pof coloured by chain, side view.
PDB entry 2pof coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Entry authors: Madegowda M, Eswaramoorthy S, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
CDP-diacylglycerol + H(2)O = CMP + phosphatidate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186850 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CDP-diacylglycerol pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 26.11 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8X7A5 (Residues: 27-251; Coverage: 90%)
Gene names: ECs4843, Z5463, cdh
Sequence domains: CDP-diacylglycerol pyrophosphatase
Structure domains: HIT family - CDH-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 37.246Å b: 65.418Å c: 80.926Å
α: 90° β: 90.21° γ: 90°
R-values:
R R work R free
0.249 0.249 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links