› 2pox

X-ray diffraction
1.95Å resolution

Dark state structure of the reversibly switchable fluorescent protein Dronpa

Released:
DOI: 10.2210/pdb2pox/pdb
PDB entry 2pox coloured by chain, front view.
PDB entry 2pox coloured by chain, side view.
PDB entry 2pox coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
Structural basis for reversible photoswitching in Dronpa.
Andresen M, Stiel AC, Trowitzsch S, Weber G, Eggeling C, Wahl MC, Hell SW, Jakobs S
Proc Natl Acad Sci U S A 104 13005-9 (2007)
PMID: 17646653

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178341 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 255 amino acids
Theoretical weight: 29.25 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5TLG6 (Residues: 1-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYC 4 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I222
Unit cell:
a: 72.894Å b: 107.504Å c: 275.663Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.216
Expression system: Escherichia coli

Quick links

Citations

33 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
32 more

10 mentions without citation

Following Optogenetic Dimerizers and Quantitative Prospects.
Niu et al. (2016)
9 more