› 2ppy

X-ray diffraction
2.16Å resolution

Crystal structure of Enoyl-CoA hydrates (gk_1992) from Geobacillus Kaustophilus HTA426

Released:
DOI: 10.2210/pdb2ppy/pdb
PDB entry 2ppy coloured by chain, front view.
PDB entry 2ppy coloured by chain, side view.
PDB entry 2ppy coloured by chain, top view.
Source organism: Geobacillus kaustophilus HTA426
Entry authors: Kanaujia SP, Jeyakanthan J, Kavyashree M, Sekar K, Ebihara A, Kuramitsu S, Shinkai A, Shiro Y, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177389 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-CoA hydratase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 265 amino acids
Theoretical weight: 29.92 KDa
Source organism: Geobacillus kaustophilus HTA426
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5KYF9 (Residues: 1-265; Coverage: 100%)
Gene name: GK1992
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PEG 6 x PEG
Ligand EDO 2 x EDO
1 modified residue:
Ligand MSE 42 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P21212
Unit cell:
a: 136.201Å b: 137.345Å c: 79.131Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.219
Expression system: Escherichia coli

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