› 2prk

X-ray diffraction
1.5Å resolution

SYNCHROTRON X-RAY DATA COLLECTION AND RESTRAINED LEAST-SQUARES REFINEMENT OF THE CRYSTAL STRUCTURE OF PROTEINASE K AT 1.5 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb2prk/pdb
PDB entry 2prk coloured by chain, front view.
PDB entry 2prk coloured by chain, side view.
PDB entry 2prk coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
Synchrotron X-ray data collection and restrained least-squares refinement of the crystal structure of proteinase K at 1.5 A resolution.
Betzel C, Pal GP, Saenger W
Acta Crystallogr B 44 ( Pt 2) 163-72 (1988)
PMID: 3271105

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase K Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 28.93 KDa
Source organism: Parengyodontium album
Expression system: Not provided
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 68.17Å b: 68.17Å c: 108.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 not available not available
Expression system: Not provided

Quick links

Citations

2 review citations

Plant alpha-amylase inhibitors and their interaction with insect alpha-amylases.
Franco et al. (2002)
1 more

13 mentions without citation

Development of a polarizable force field for proteins via ab initio quantum chemistry: first generation model and gas phase tests.
Kaminski et al. (2002)
12 more