› 2ps1

X-ray diffraction
1.75Å resolution

S. cerevisiae orotate phosphoribosyltransferase complexed with orotic acid and PRPP

Released:
DOI: 10.2210/pdb2ps1/pdb
PDB entry 2ps1 coloured by chain, front view.
PDB entry 2ps1 coloured by chain, side view.
PDB entry 2ps1 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Ternary complex formation and induced asymmetry in orotate phosphoribosyltransferase.
González-Segura L, Witte JF, McClard RW, Hurley TD
Biochemistry 46 14075-86 (2007)
PMID: 18020427

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orotate phosphoribosyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 226 amino acids
Theoretical weight: 24.69 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P13298 (Residues: 1-226; Coverage: 100%)
Gene names: PYR5, URA5, YM8339.13, YML106W
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ORO 2 x ORO
Ligand PRP 2 x PRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 41.788Å b: 50.051Å c: 50.078Å
α: 90.59° β: 105.88° γ: 92.99°
R-values:
R R work R free
0.195 0.194 0.217
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.
Hove-Jensen et al. (2017)
2 more

4 mentions without citation

Leaving group activation and pyrophosphate ionic state at the catalytic site of Plasmodium falciparum orotate phosphoribosyltransferase.
Zhang et al. (2010)
3 more