› 2psz

X-ray diffraction
2Å resolution

Structure of the PTP-like Phytase expressed by Selenomonas ruminantium at low ionic strength

Released:
DOI: 10.2210/pdb2psz/pdb
PDB entry 2psz coloured by chain, front view.
PDB entry 2psz coloured by chain, side view.
PDB entry 2psz coloured by chain, top view.
Source organism: Selenomonas ruminantium
Primary publication:
Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr.
Gruninger RJ, Selinger LB, Mosimann SC
FEBS J 275 3783-92 (2008)
PMID: 18573100

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182145 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine specific protein phosphatases domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 38.99 KDa
Source organism: Selenomonas ruminantium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7WUJ1 (Residues: 28-346; Coverage: 100%)
Gene name: phyA
Sequence domains: Inositol hexakisphosphate
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 45.917Å b: 137.698Å c: 80.096Å
α: 90° β: 102.8° γ: 90°
R-values:
R R work R free
0.188 0.183 0.214
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Protein tyrosine phosphatase structure-function relationships in regulation and pathogenesis.
Böhmer et al. (2013)
8 other articles