› 2pt7

X-ray diffraction
2.4Å resolution

Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)

Released:
DOI: 10.2210/pdb2pt7/pdb
Model geometry
Fit model/data
PDB entry 2pt7 coloured by chain, front view.
PDB entry 2pt7 coloured by chain, side view.
PDB entry 2pt7 coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase.
Hare S, Fischer W, Williams R, Terradot L, Bayliss R, Haas R, Waksman G
EMBO J 26 4926-34 (2007)
PMID: 17972918

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127808 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bacterial type II secretion system protein E domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 330 amino acids
Theoretical weight: 37.63 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7BK04 (Residues: 1-330; Coverage: 100%)
Gene names: ACM37_01770, C2842_02730, C2R72_03110, CV728_03100, EC547_07075, EGM89_00300, F7219_04320, HP0525, RGC53_05420, SE88_02715, cag-alfa, cag-alpha, cagAlpha, cagalpha, virB11
Sequence domains: Type II/IV secretion system protein
Structure domains:
RNA-binding protein KhpB N-terminal domain-containing protein Chains: G, H
Molecule details ›
Chains: G, H
Length: 152 amino acids
Theoretical weight: 17.58 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O25990 (Residues: 113-264; Coverage: 58%)
Gene name: HP_1451
Sequence domains: HP1451 C-terminal domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 86.851Å b: 86.9Å c: 104.129Å
α: 111.04° β: 95.98° γ: 104.94°
R-values:
R R work R free
0.232 0.23 0.27
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
4 more

7 mentions without citation

Recent advances in the structural and molecular biology of type IV secretion systems.
Trokter et al. (2014)
6 more