› 2ptd

X-ray diffraction
2Å resolution

PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C MUTANT D198E

Released:
DOI: 10.2210/pdb2ptd/pdb
PDB entry 2ptd coloured by chain, front view.
PDB entry 2ptd coloured by chain, side view.
PDB entry 2ptd coloured by chain, top view.
Source organism: Bacillus cereus
Primary publication:
Probing the roles of active site residues in phosphatidylinositol-specific phospholipase C from Bacillus cereus by site-directed mutagenesis.
Gässler CS, Ryan M, Liu T, Griffith OH, Heinz DW
Biochemistry 36 12802-13 (1997)
PMID: 9335537

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 34.58 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14262 (Residues: 32-329; Coverage: 100%)
Sequence domains: Phosphatidylinositol-specific phospholipase C, X domain
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 45.6Å b: 45.9Å c: 161.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.271
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Families of phosphoinositide-specific phospholipase C: structure and function.
Katan M. (1998)
1 more

2 mentions without citation

The role of hydrophobic interactions in positioning of peripheral proteins in membranes.
Lomize et al. (2007)
1 more