› 2puo

X-ray diffraction
1.7Å resolution

Crystal srtucture of the NEM modified ferredoxin:thioredoxin reductase

Released:
DOI: 10.2210/pdb2puo/pdb
PDB entry 2puo coloured by chain, front view.
PDB entry 2puo coloured by chain, side view.
PDB entry 2puo coloured by chain, top view.
Source organism: Synechocystis sp.
Primary publication:
Structural snapshots along the reaction pathway of ferredoxin-thioredoxin reductase.
Dai S, Friemann R, Glauser DA, Bourquin F, Manieri W, Schürmann P, Eklund H
Nature 448 92-6 (2007)
PMID: 17611542

Function and Biology Details

Reaction catalysed: 
2 reduced ferredoxin + thioredoxin disulfide = 2 oxidized ferredoxin + thioredoxin + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176276 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ferredoxin-thioredoxin reductase, catalytic chain Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.45 KDa
Source organism: Synechocystis sp.
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q55389 (Residues: 8-116; Coverage: 92%)
Gene names: ftrC, sll0554
Sequence domains: Ferredoxin thioredoxin reductase catalytic beta chain
Structure domains: Ferredoxin thioredoxin reductase catalytic beta subunit
Ferredoxin-thioredoxin reductase, variable chain Chain: B
Molecule details ›
Chain: B
Length: 73 amino acids
Theoretical weight: 8.33 KDa
Source organism: Synechocystis sp.
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q55781 (Residues: 1-73; Coverage: 97%)
Gene names: ftrV, ssr0330
Sequence domains: Ferredoxin thioredoxin reductase variable alpha chain
Structure domains: SH3 type barrels.

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand SF4 1 x SF4
Ligand NEQ 1 x NEQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 44.65Å b: 53.595Å c: 79.393Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.255
Expression system: Escherichia coli BL21

Quick links

Citations

15 review citations

The ferredoxin/thioredoxin system of oxygenic photosynthesis.
Schürmann et al. (2008)
14 more

1 mention without citation

SF2312 is a natural phosphonate inhibitor of enolase.
Leonard et al. (2016)