› 2puw

X-ray diffraction
3.15Å resolution

The crystal structure of isomerase domain of glucosamine-6-phosphate synthase from Candida albicans

Released:
DOI: 10.2210/pdb2puw/pdb
PDB entry 2puw coloured by chain, front view.
PDB entry 2puw coloured by chain, side view.
PDB entry 2puw coloured by chain, top view.
Source organism: Candida albicans SC5314
Primary publication:
The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans.
Raczynska J, Olchowy J, Konariev PV, Svergun DI, Milewski S, Rypniewski W
J Mol Biol 372 672-88 (2007)
PMID: 17681543

Function and Biology Details

Reaction catalysed: 
L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156861 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] Chains: A, B
Molecule details ›
Chains: A, B
Length: 367 amino acids
Theoretical weight: 40.61 KDa
Source organism: Candida albicans SC5314
Expression system: Escherichia coli
UniProt:
  • Canonical: P53704 (Residues: 347-713; Coverage: 52%)
Gene names: CAALFM_C302280CA, CaO19.1618, GFA1
Sequence domains: SIS domain
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1

Ligands and Environments

2 bound ligands:
Ligand BG6 2 x BG6
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: I4
Unit cell:
a: 148.864Å b: 148.864Å c: 102.878Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.23 0.279
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
5 more

2 mentions without citation

Functional co-evolutionary study of glucosamine-6-phosphate synthase in mycoses causing fungi.
Banerjee et al. (2011)
1 more