› 2pvs

X-ray diffraction
3Å resolution

Structure of human pancreatic lipase related protein 2 mutant N336Q

Released:
DOI: 10.2210/pdb2pvs/pdb
PDB entry 2pvs coloured by chain, front view.
PDB entry 2pvs coloured by chain, side view.
PDB entry 2pvs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human pancreatic lipase-related protein 2 with the lid in an open conformation.
Eydoux C, Spinelli S, Davis TL, Walker JR, Seitova A, Dhe-Paganon S, De Caro A, Cambillau C, Carrière F
Biochemistry 47 9553-64 (2008)
PMID: 18702514

Function and Biology Details

Reactions catalysed: 
1,2-diacyl-3-beta-D-galactosyl-sn-glycerol + 2 H(2)O = 3-beta-D-galactosyl-sn-glycerol + 2 carboxylates
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-157007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pancreatic lipase-related protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 452 amino acids
Theoretical weight: 50.18 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P54317 (Residues: 18-469; Coverage: 100%)
Gene names: PLRP2, PNLIPRP2
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: I4122
Unit cell:
a: 216.181Å b: 216.181Å c: 123.672Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.249
Expression system: Komagataella pastoris

Quick links

Citations

4 review citations

Physiological parameters governing the action of pancreatic lipase.
Brownlee et al. (2010)
3 more

4 mentions without citation

Current Knowledge on Mammalian Phospholipase A1, Brief History, Structures, Biochemical and Pathophysiological Roles.
Yaginuma et al. (2022)
3 more