› 2pvu

X-ray diffraction
1.79Å resolution

Crystal structures of the arginine-, lysine-, histidine-binding protein ArtJ from the thermophilic bacterium Geobacillus stearothermophilus

Released:
DOI: 10.2210/pdb2pvu/pdb
PDB entry 2pvu coloured by chain, front view.
PDB entry 2pvu coloured by chain, side view.
PDB entry 2pvu coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structures and mutational analysis of the arginine-, lysine-, histidine-binding protein ArtJ from Geobacillus stearothermophilus. Implications for interactions of ArtJ with its cognate ATP-binding cassette transporter, Art(MP)2.
Vahedi-Faridi A, Eckey V, Scheffel F, Alings C, Landmesser H, Schneider E, Saenger W
J Mol Biol 375 448-59 (2008)
PMID: 18022195

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112150 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ArtJ Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 29.69 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VWX8 (Residues: 1-252; Coverage: 100%)
Sequence domains: Bacterial extracellular solute-binding proteins, family 3
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand LYS 1 x LYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2221
Unit cell:
a: 68.268Å b: 73.887Å c: 102.681Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.174 0.224
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Canonical and ECF-type ATP-binding cassette importers in prokaryotes: diversity in modular organization and cellular functions.
Eitinger et al. (2011)
2 more

3 mentions without citation

Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies.
Ghosh et al. (2022)
2 more