› 2pw6

X-ray diffraction
2.27Å resolution

Crystal structure of uncharacterized protein JW3007 from Escherichia coli K12

Released:
DOI: 10.2210/pdb2pw6/pdb
PDB entry 2pw6 coloured by chain, front view.
PDB entry 2pw6 coloured by chain, side view.
PDB entry 2pw6 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Newton MG, Takagi Y, Ebihara A, Shinkai A, Kuramitsu S, Yokayama S, Li Y, Chen L, Zhu J, Ruble J, Liu ZJ, Rose JP, Wang B-C, Southeast Collaboratory for Structural Genomics (SECSG), RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
L-dopa + O(2) = 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150170 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4,5-DOPA dioxygenase extradiol Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 30.49 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P24197 (Residues: 1-271; Coverage: 100%)
Gene names: JW3007, b3039, ygiD
Sequence domains: Catalytic LigB subunit of aromatic ring-opening dioxygenase
Structure domains: LigB-like

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3121
Unit cell:
a: 83.94Å b: 83.94Å c: 85.509Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.235 0.267
Expression system: Escherichia coli

Quick links