› 2pwy

X-ray diffraction
1.7Å resolution

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(58) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (adenine(58)-N(1))-methyltransferase TrmI Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 28.91 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GBB2 (Residues: 1-255; Coverage: 100%)
Gene names: TT_C0244, trmI
Sequence domains: tRNA methyltransferase complex GCD14 subunit
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2221
Unit cell:
a: 90.44Å b: 96.795Å c: 140.584Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.209
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Methylated nucleosides in tRNA and tRNA methyltransferases.
Hori H. (2014)
12 more

5 mentions without citation

m1A Post-Transcriptional Modification in tRNAs.
Oerum et al. (2017)
4 more