› 2px7

X-ray diffraction
2.2Å resolution

Crystal structure of 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2px7/pdb
PDB entry 2px7 coloured by chain, front view.
PDB entry 2px7 coloured by chain, side view.
PDB entry 2px7 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Chen L, Tsukuda M, Ebihara A, Shinkai A, Kuramitsu S, Yokoyama S, Chen L-Q, Liu Z-J, Lee D, Chang S-H, Nguyen D, Rose JP, Wang B-C, Southeast Collaboratory for Structural Genomics (SECSG), RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178223 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 236 amino acids
Theoretical weight: 25.23 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLX2 (Residues: 1-213; Coverage: 100%)
Gene names: TTHA0171, ispD
Sequence domains: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P61
Unit cell:
a: 104.084Å b: 104.084Å c: 107.76Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.227 0.248
Expression system: Escherichia coli

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