› 2q0e

X-ray diffraction
2.1Å resolution

Terminal uridylyl transferase 4 from Trypanosoma brucei with bound GTP

Released:
DOI: 10.2210/pdb2q0e/pdb
PDB entry 2q0e coloured by chain, front view.
PDB entry 2q0e coloured by chain, side view.
PDB entry 2q0e coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
Dual role of the RNA substrate in selectivity and catalysis by terminal uridylyl transferases.
Stagno J, Aphasizheva I, Aphasizhev R, Luecke H
Proc Natl Acad Sci U S A 104 14634-9 (2007)
PMID: 17785418

Function and Biology Details

Reaction catalysed: 
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 39.88 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q381M1 (Residues: 1-333; Coverage: 100%)
Gene names: TUT4, Tb11.01.7300
Sequence domains: Cid1 family poly A polymerase

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 80.788Å b: 41.917Å c: 106.388Å
α: 90° β: 93.34° γ: 90°
R-values:
R R work R free
0.234 0.232 0.28
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

Uridine insertion/deletion editing in trypanosomes: a playground for RNA-guided information transfer.
Aphasizhev et al. (2011)
7 more

2 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
1 more