› 2q0f

X-ray diffraction
2.4Å resolution

Terminal uridylyl transferase 4 from Trypanosoma brucei with bound UTP and UMP

Released:
DOI: 10.2210/pdb2q0f/pdb
PDB entry 2q0f coloured by chain, front view.
PDB entry 2q0f coloured by chain, side view.
PDB entry 2q0f coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
Dual role of the RNA substrate in selectivity and catalysis by terminal uridylyl transferases.
Stagno J, Aphasizheva I, Aphasizhev R, Luecke H
Proc Natl Acad Sci U S A 104 14634-9 (2007)
PMID: 17785418

Function and Biology Details

Reaction catalysed: 
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 39.88 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q381M1 (Residues: 1-333; Coverage: 100%)
Gene names: TUT4, Tb11.01.7300
Sequence domains: Cid1 family poly A polymerase

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand UTP 1 x UTP
Ligand U5P 2 x U5P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P21
Unit cell:
a: 80.62Å b: 41.924Å c: 106.017Å
α: 90° β: 94.37° γ: 90°
R-values:
R R work R free
0.226 0.223 0.293
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Uridine insertion/deletion editing in trypanosomes: a playground for RNA-guided information transfer.
Aphasizhev et al. (2011)
6 more

4 mentions without citation

Determinants of substrate specificity in RNA-dependent nucleotidyl transferases.
Martin et al. (2008)
3 more