› 2q10

X-ray diffraction
1.75Å resolution

RESTRICTION ENDONUCLEASE BcnI (WILD TYPE)-COGNATE DNA SUBSTRATE COMPLEX

Released:
DOI: 10.2210/pdb2q10/pdb
PDB entry 2q10 coloured by chain, front view.
PDB entry 2q10 coloured by chain, side view.
PDB entry 2q10 coloured by chain, top view.
Source organism: Brevibacillus centrosporus
Primary publication:
Monomeric restriction endonuclease BcnI in the apo form and in an asymmetric complex with target DNA.
Sokolowska M, Kaus-Drobek M, Czapinska H, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M
J Mol Biol 369 722-34 (2007)
PMID: 17445830

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115795 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
MvaI/BcnI restriction endonuclease domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 27.33 KDa
Source organism: Brevibacillus centrosporus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RNV8 (Residues: 1-238; Coverage: 100%)
Gene name: bcnIR
Sequence domains: MvaI/BcnI restriction endonuclease family
Structure domains:
DNA (5'-D(*AP*AP*CP*CP*CP*GP*GP*AP*GP*AP*C)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 11 nucleotides
Theoretical weight: 3.35 KDa
Source organism: Brevibacillus centrosporus
Expression system: Not provided
DNA (5'-D(*CP*TP*CP*CP*GP*GP*GP*TP*TP*GP*T)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 11 nucleotides
Theoretical weight: 3.36 KDa
Source organism: Brevibacillus centrosporus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P21212
Unit cell:
a: 81.354Å b: 87.551Å c: 79.162Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.213
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
1 more

4 mentions without citation

Computational approaches to predicting the impact of novel bases on RNA structure and stability.
Harrison et al. (2013)
3 more