› 2q1r

X-ray diffraction
1.12Å resolution

Crystal Structure Analysis of the RNA Dodecamer CGCGAAUUAGCG, with a G-A mismatch.

Released:
DOI: 10.2210/pdb2q1r/pdb
PDB entry 2q1r coloured by chain, front view.
PDB entry 2q1r coloured by chain, side view.
PDB entry 2q1r coloured by chain, top view.
Source organism: Unidentified
Primary publication:
Crystal structure, stability and in vitro RNAi activity of oligoribonucleotides containing the ribo-difluorotoluyl nucleotide: insights into substrate requirements by the human RISC Ago2 enzyme.
Li F, Pallan PS, Maier MA, Rajeev KG, Mathieu SL, Kreutz C, Fan Y, Sanghvi J, Micura R, Rozners E, Manoharan M, Egli M
Nucleic Acids Res 35 6424-38 (2007)
PMID: 17881374

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196568 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (5'-R(*CP*GP*CP*GP*AP*AP*UP*UP*AP*GP*CP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 12 nucleotides
Theoretical weight: 3.85 KDa
Source organism: Unidentified
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: C2
Unit cell:
a: 41.339Å b: 34.886Å c: 32.118Å
α: 90° β: 129.32° γ: 90°
R-values:
R R work R free
0.149 0.142 0.182
Expression system: Not provided

Quick links

Citations

11 review citations

Carbon-oxygen hydrogen bonding in biological structure and function.
Horowitz et al. (2012)
10 more

4 mentions without citation

Crystal structure, stability and in vitro RNAi activity of oligoribonucleotides containing the ribo-difluorotoluyl nucleotide: insights into substrate requirements by the human RISC Ago2 enzyme.
Li et al. (2007)
3 more