› 2q3f

X-ray diffraction
2.1Å resolution

X-ray crystal structure of putative human Ras-related GTP binding D in complex with GMPPNP

Released:
DOI: 10.2210/pdb2q3f/pdb
PDB entry 2q3f coloured by chain, front view.
PDB entry 2q3f coloured by chain, side view.
PDB entry 2q3f coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Mulichak AM, Rabeh WM, Tempel W, Nedyalkova L, Landry R, Arrowsmith CH, Edwards AM, Sundstrom M, Weigelt J, Keefe LJ, Bochkarev A, Park H, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related GTP-binding protein D Chains: A, B
Molecule details ›
Chains: A, B
Length: 181 amino acids
Theoretical weight: 21.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NQL2 (Residues: 59-239; Coverage: 45%)
Gene name: RRAGD
Sequence domains: Gtr1/RagA G protein conserved region
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GNP 2 x GNP
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 47.73Å b: 67.13Å c: 66.36Å
α: 90° β: 110.92° γ: 90°
R-values:
R R work R free
0.223 0.221 0.268
Expression system: Escherichia coli BL21(DE3)

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