› 2q3l

X-ray diffraction
2.25Å resolution

CRYSTAL STRUCTURE OF AN UNCHARACTERIZED PROTEIN FROM DUF3478 FAMILY WITH A SPOIIAA-LIKE FOLD (SHEW_3102) FROM SHEWANELLA LOIHICA PV-4 AT 2.25 A RESOLUTION

Released:
DOI: 10.2210/pdb2q3l/pdb
PDB entry 2q3l coloured by chain, front view.
PDB entry 2q3l coloured by chain, side view.
PDB entry 2q3l coloured by chain, top view.
Source organism: Shewanella loihica PV-4
Primary publication:
Open and closed conformations of two SpoIIAA-like proteins (YP_749275.1 and YP_001095227.1) provide insights into membrane association and ligand binding.
Kumar A, Lomize A, Jin KK, Carlton D, Miller MD, Jaroszewski L, Abdubek P, Astakhova T, Axelrod HL, Chiu HJ, Clayton T, Das D, Deller MC, Duan L, Feuerhelm J, Grant JC, Grzechnik A, Han GW, Klock HE, Knuth MW, Kozbial P, Krishna SS, Marciano D, McMullan D, Morse AT, Nigoghossian E, Okach L, Reyes R, Rife CL, Sefcovic N, Tien HJ, Trame CB, van den Bedem H, Weekes D, Xu Q, Hodgson KO, Wooley J, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Wilson IA
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 1245-53 (2010)
PMID: 20944218

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
STAS/SEC14 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 14.27 KDa
Source organism: Shewanella loihica PV-4
Expression system: Escherichia coli
UniProt:
  • Canonical: A3QHM0 (Residues: 1-125; Coverage: 100%)
Gene name: Shew_3102
Sequence domains: SpoIIAA-like
Structure domains: SpoIIaa-like domains

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand CL 2 x CL
Ligand MPD 9 x MPD
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2221
Unit cell:
a: 40.387Å b: 113.963Å c: 130.692Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.239
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structure of MlaFB uncovers novel mechanisms of ABC transporter regulation.
Kolich et al. (2020)

7 mentions without citation

Structural classification of proteins and structural genomics: new insights into protein folding and evolution.
Andreeva et al. (2010)
6 more