› 2q5e

X-ray diffraction
2.51Å resolution

Crystal structure of human carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2

Released:
DOI: 10.2210/pdb2q5e/pdb
PDB entry 2q5e coloured by chain, front view.
PDB entry 2q5e coloured by chain, side view.
PDB entry 2q5e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural genomics of protein phosphatases.
Almo SC, Bonanno JB, Sauder JM, Emtage S, Dilorenzo TP, Malashkevich V, Wasserman SR, Swaminathan S, Eswaramoorthy S, Agarwal R, Kumaran D, Madegowda M, Ragumani S, Patskovsky Y, Alvarado J, Ramagopal UA, Faber-Barata J, Chance MR, Sali A, Fiser A, Zhang ZY, Lawrence DS, Burley SK
J Struct Funct Genomics 8 121-40 (2007)
PMID: 18058037

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127018 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 187 amino acids
Theoretical weight: 21.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14595 (Residues: 87-271; Coverage: 68%)
Gene names: CTDSP2, NIF2, OS4, SCP2
Sequence domains: NLI interacting factor-like phosphatase
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

1 bound ligand:
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 108.643Å b: 117.637Å c: 170.134Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

Aiding and abetting roles of NOX oxidases in cellular transformation.
Block et al. (2012)
22 more

4 mentions without citation

Structural genomics of protein phosphatases.
Almo et al. (2007)
3 more