› 2q66

X-ray diffraction
1.8Å resolution

Structure of Yeast Poly(A) Polymerase with ATP and oligo(A)

Released:
DOI: 10.2210/pdb2q66/pdb
PDB entry 2q66 coloured by chain, front view.
PDB entry 2q66 coloured by chain, side view.
PDB entry 2q66 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Mechanism of poly(A) polymerase: structure of the enzyme-MgATP-RNA ternary complex and kinetic analysis.
Balbo PB, Bohm A
Structure 15 1117-31 (2007)
PMID: 17850751

Function and Biology Details

Reaction catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151508 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Poly(A) polymerase Chain: A
Molecule details ›
Chain: A
Length: 525 amino acids
Theoretical weight: 60 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P29468 (Residues: 5-529; Coverage: 92%)
Gene names: PAP1, YKR002W
Sequence domains:
Structure domains:
5'-R(P*AP*AP*AP*AP*A)-3' Chain: X
Molecule details ›
Chain: X
Length: 5 nucleotides
Theoretical weight: 1.6 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand EDO 17 x EDO
Ligand MG 1 x MG
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 67.633Å b: 85.907Å c: 107.469Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.225
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 review citations

Protein factors in pre-mRNA 3'-end processing.
Mandel et al. (2008)
13 more

21 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
20 more