2q67

X-ray diffraction
2.3Å resolution

Crystal Structure of Nak channel D66A mutant

Released:
Source organism: Bacillus cereus
Primary publication:
Structural insight into Ca2+ specificity in tetrameric cation channels.
Proc Natl Acad Sci U S A 104 15334-9 (2007)
PMID: 17878296

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-182541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 114 amino acids
Theoretical weight: 12.79 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81HW2 (Residues: 1-110; Coverage: 97%)
Gene name: BC_0669
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2221
Unit cell:
a: 82.23Å b: 85.27Å c: 130.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.266
Expression system: Escherichia coli