› 2q69

X-ray diffraction
2.4Å resolution

Crystal Structure of Nak channel D66N mutant

Released:
DOI: 10.2210/pdb2q69/pdb
PDB entry 2q69 coloured by chain, front view.
PDB entry 2q69 coloured by chain, side view.
PDB entry 2q69 coloured by chain, top view.
Source organism: Bacillus cereus
Primary publication:
Structural insight into Ca2+ specificity in tetrameric cation channels.
Alam A, Shi N, Jiang Y
Proc Natl Acad Sci U S A 104 15334-9 (2007)
PMID: 17878296

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 114 amino acids
Theoretical weight: 12.83 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81HW2 (Residues: 1-110; Coverage: 97%)
Gene name: BC_0669
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 82.345Å b: 85.71Å c: 130.648Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.267
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Molecular bases of NMDA receptor subtype-dependent properties.
Glasgow et al. (2015)
3 more

1 mention without citation

Structural insight into Ca2+ specificity in tetrameric cation channels.
Alam et al. (2007)