› 2q6n

X-ray diffraction
3.2Å resolution

Structure of Cytochrome P450 2B4 with Bound 1-(4-cholorophenyl)imidazole

Released:
DOI: 10.2210/pdb2q6n/pdb
PDB entry 2q6n coloured by chain, front view.
PDB entry 2q6n coloured by chain, side view.
PDB entry 2q6n coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Structural and thermodynamic consequences of 1-(4-chlorophenyl)imidazole binding to cytochrome P450 2B4.
Zhao Y, Sun L, Muralidhara BK, Kumar S, White MA, Stout CD, Halpert JR
Biochemistry 46 11559-67 (2007)
PMID: 17887776

Function and Biology Details

Reaction catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132309 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 2B4 Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 478 amino acids
Theoretical weight: 54.45 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00178 (Residues: 20-491; Coverage: 96%)
Gene name: CYP2B4
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 7 x HEM
1 bound ligand:
Ligand 1CI 7 x 1CI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 140Å b: 147.31Å c: 238.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.311
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Enzyme promiscuity: a mechanistic and evolutionary perspective.
Khersonsky et al. (2010)
8 more

12 mentions without citation

Structural features of cytochromes P450 and ligands that affect drug metabolism as revealed by X-ray crystallography and NMR.
Gay et al. (2010)
11 more