› 2q6q

X-ray diffraction
1.97Å resolution

Crystal structure of Spc42p, a critical component of spindle pole body in budding yeast

Released:
DOI: 10.2210/pdb2q6q/pdb
PDB entry 2q6q coloured by chain, front view.
PDB entry 2q6q coloured by chain, side view.
PDB entry 2q6q coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Analysis of coiled-coil interactions between core proteins of the spindle pole body.
Zizlsperger N, Malashkevich VN, Pillay S, Keating AE
Biochemistry 47 11858-68 (2008)
PMID: 18850724

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153118 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spindle pole body component SPC42 Chains: A, B
Molecule details ›
Chains: A, B
Length: 74 amino acids
Theoretical weight: 8.86 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P36094 (Residues: 65-138; Coverage: 20%)
Gene names: SPC42, YKL042W, YKL255
Sequence domains: Spindle pole body component Spc42p
Structure domains: Geminin coiled-coil domain

Ligands and Environments

2 bound ligands:
Ligand CO 3 x CO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 42.86Å b: 57.79Å c: 57.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.212 0.297
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Protein binding specificity versus promiscuity.
Schreiber et al. (2011)
3 more

2 mentions without citation

Structural attributes for the recognition of weak and anomalous regions in coiled-coils of myosins and other motor proteins.
Sunitha et al. (2012)
1 more