› 2q6v

X-ray diffraction
2.28Å resolution

Crystal Structure of GumK in complex with UDP

Released:
DOI: 10.2210/pdb2q6v/pdb
PDB entry 2q6v coloured by chain, front view.
PDB entry 2q6v coloured by chain, side view.
PDB entry 2q6v coloured by chain, top view.
Source organism: Xanthomonas campestris pv. campestris
Primary publication:
Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase.
Barreras M, Salinas SR, Abdian PL, Kampel MA, Ielpi L
J Biol Chem 283 25027-35 (2008)
PMID: 18596046

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucuronate + alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol = UDP + beta-D-GlcA-(1->2)-alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucuronate:glycolipid 2-beta-glucuronosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 45.34 KDa
Source organism: Xanthomonas campestris pv. campestris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GCH2 (Residues: 1-400; Coverage: 100%)
Gene name: gumK
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand UDP 1 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P6522
Unit cell:
a: 121.21Å b: 121.21Å c: 170.724Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.175 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Bacterial polymers: biosynthesis, modifications and applications.
Rehm BH. (2010)
5 more

3 mentions without citation

Structure-function relationships of membrane-associated GT-B glycosyltransferases.
Albesa-Jové et al. (2014)
2 more