› 2q7v

X-ray diffraction
1.9Å resolution

Crystal Structure of Deinococcus Radiodurans Thioredoxin Reductase

Released:
DOI: 10.2210/pdb2q7v/pdb
PDB entry 2q7v coloured by chain, front view.
PDB entry 2q7v coloured by chain, side view.
PDB entry 2q7v coloured by chain, top view.
Source organism: Deinococcus radiodurans
Primary publication:
Thioredoxin system from Deinococcus radiodurans.
Obiero J, Pittet V, Bonderoff SA, Sanders DA
J Bacteriol 192 494-501 (2010)
PMID: 19933368

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 325 amino acids
Theoretical weight: 35.07 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RSY7 (Residues: 1-325; Coverage: 100%)
Gene name: DR_1982
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P3221
Unit cell:
a: 83.994Å b: 83.994Å c: 160.11Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.188 0.24
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Oxidative stress resistance in Deinococcus radiodurans.
Slade et al. (2011)
2 more

7 mentions without citation

Structural features and functional implications of proteins enabling the robustness of Deinococcus radiodurans.
Chen et al. (2020)
6 more