› 2q83

X-ray diffraction
2.5Å resolution

Crystal structure of ytaA (2635576) from Bacillus subtilis at 2.50 A resolution

Released:
DOI: 10.2210/pdb2q83/pdb
PDB entry 2q83 coloured by chain, front view.
PDB entry 2q83 coloured by chain, side view.
PDB entry 2q83 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Genomics, evolution, and crystal structure of a new family of bacterial spore kinases.
Scheeff ED, Axelrod HL, Miller MD, Chiu HJ, Deacon AM, Wilson IA, Manning G
Proteins 78 1470-82 (2010)
PMID: 20077512

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128573 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spore coat protein I Chains: A, B
Molecule details ›
Chains: A, B
Length: 346 amino acids
Theoretical weight: 40.4 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O34656 (Residues: 13-357; Coverage: 97%)
Gene names: BSU30920, cotI, ytaA
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ADN 2 x ADN
Ligand UNL 2 x UNL
Ligand EDO 8 x EDO
Ligand SO4 2 x SO4
Ligand CIT 1 x CIT
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6422
Unit cell:
a: 172.999Å b: 172.999Å c: 192.574Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.198 0.21
Expression system: Escherichia coli

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Citations

3 review citations

Recent progress in Bacillus subtilis sporulation.
Higgins et al. (2012)
2 more

1 mention without citation

Genomics, evolution, and crystal structure of a new family of bacterial spore kinases.
Scheeff et al. (2010)