› 2q8p

X-ray diffraction
1.95Å resolution

Crystal Structure of selenomethionine labelled S. aureus IsdE complexed with heme

Released:
DOI: 10.2210/pdb2q8p/pdb
PDB entry 2q8p coloured by chain, front view.
PDB entry 2q8p coloured by chain, side view.
PDB entry 2q8p coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus N315
Primary publication:
Heme coordination by Staphylococcus aureus IsdE.
Grigg JC, Vermeiren CL, Heinrichs DE, Murphy MEP
J Biol Chem 282 28815-28822 (2007)
PMID: 17666394

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
High-affinity heme uptake system protein IsdE Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 30.11 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7A652 (Residues: 32-289; Coverage: 95%)
Gene names: SA0980, isdE, sirF
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P43212
Unit cell:
a: 63.527Å b: 63.527Å c: 144.247Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.263
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

Iron in infection and immunity.
Cassat et al. (2013)
16 more

2 mentions without citation

Specificity of Staphyloferrin B recognition by the SirA receptor from Staphylococcus aureus.
Grigg et al. (2010)
1 more