› 2q9e

Hybrid

Structure of spin-labeled T4 lysozyme mutant S44R1

Released:
DOI: 10.2210/pdb2q9e/pdb
PDB entry 2q9e coloured by chain, front view.
PDB entry 2q9e coloured by chain, side view.
PDB entry 2q9e coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Structural determinants of nitroxide motion in spin-labeled proteins: solvent-exposed sites in helix B of T4 lysozyme.
Guo Z, Cascio D, Hideg K, Hubbell WL
Protein Sci 17 228-39 (2008)
PMID: 18096642

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 164 amino acids
Theoretical weight: 18.6 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00720 (Residues: 1-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

2 bound ligands:
Ligand MTN 3 x MTN
Ligand HED 3 x HED
No modified residues

Experiments and Validation Details

Experimental Method: X-ray diffraction, EPR
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 57.989Å b: 67.14Å c: 165.919Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Fibrils with parallel in-register structure constitute a major class of amyloid fibrils: molecular insights from electron paramagnetic resonance spectroscopy.
Margittai et al. (2008)
4 more

7 mentions without citation

MtsslWizard: In Silico Spin-Labeling and Generation of Distance Distributions in PyMOL.
Hagelueken et al. (2012)
6 more