› 2q9z

X-ray diffraction
2.95Å resolution

Trichodiene synthase: Complex with inorganic pyrophosphate resulting from the reaction with 2-fluorofarnesyl diphosphate

Released:
DOI: 10.2210/pdb2q9z/pdb
PDB entry 2q9z coloured by chain, front view.
PDB entry 2q9z coloured by chain, side view.
PDB entry 2q9z coloured by chain, top view.
Source organism: Fusarium sporotrichioides
Primary publication:
Exploring biosynthetic diversity with trichodiene synthase.
Vedula LS, Zhao Y, Coates RM, Koyama T, Cane DE, Christianson DW
Arch Biochem Biophys 466 260-6 (2007)
PMID: 17678871

Function and Biology Details

Reaction catalysed: 
(2E,6E)-farnesyl diphosphate = trichodiene + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trichodiene synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 44.05 KDa
Source organism: Fusarium sporotrichioides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13513 (Residues: 1-374; Coverage: 100%)
Gene names: TOX 5, TRI5
Sequence domains: Trichodiene synthase (TRI5)
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

3 bound ligands:
Ligand EDO 2 x EDO
Ligand MG 3 x MG
Ligand POP 1 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P3121
Unit cell:
a: 122.191Å b: 122.191Å c: 150.969Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Structural and Chemical Biology of Terpenoid Cyclases.
Christianson DW. (2017)
3 more

3 mentions without citation

Sesquiterpene synthases Cop4 and Cop6 from Coprinus cinereus: catalytic promiscuity and cyclization of farnesyl pyrophosphate geometric isomers.
Lopez-Gallego et al. (2010)
2 more