› 2qa5

X-ray diffraction
3.4Å resolution

Crystal structure of Sept2 G-domain

Released:
DOI: 10.2210/pdb2qa5/pdb
PDB entry 2qa5 coloured by chain, front view.
PDB entry 2qa5 coloured by chain, side view.
PDB entry 2qa5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into filament formation by mammalian septins.
Sirajuddin M, Farkasovsky M, Hauer F, Kühlmann D, Macara IG, Weyand M, Stark H, Wittinghofer A
Nature 449 311-5 (2007)
PMID: 17637674

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Septin-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 36.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15019 (Residues: 1-315; Coverage: 87%)
Gene names: DIFF6, KIAA0158, NEDD5, SEPT2, SEPTIN2
Sequence domains: Septin
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 162.96Å b: 53.92Å c: 110.27Å
α: 90° β: 128.37° γ: 90°
R-values:
R R work R free
0.281 0.28 0.314
Expression system: Escherichia coli

Quick links

Citations

64 review citations

Septins: the fourth component of the cytoskeleton.
Mostowy et al. (2012)
63 more

11 mentions without citation

Septin structure and filament assembly.
Valadares et al. (2017)
10 more