› 2qb7

X-ray diffraction
1.6Å resolution

Saccharomyces cerevisiae cytosolic exopolyphosphatase, phosphate complex

Released:
DOI: 10.2210/pdb2qb7/pdb
PDB entry 2qb7 coloured by chain, front view.
PDB entry 2qb7 coloured by chain, side view.
PDB entry 2qb7 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure of the cytosolic exopolyphosphatase from Saccharomyces cerevisiae reveals the basis for substrate specificity.
Ugochukwu E, Lovering AL, Mather OC, Young TW, White SA
J Mol Biol 371 1007-21 (2007)
PMID: 17599355

Function and Biology Details

Reaction catalysed: 
(Polyphosphate)(n) + H(2)O = (polyphosphate)(n-1) + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 45.11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38698 (Residues: 1-397; Coverage: 100%)
Gene names: PPX1, YHR201C
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CO 4 x CO
Ligand PO4 6 x PO4
Ligand ACT 3 x ACT
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 79.992Å b: 83.039Å c: 118.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The long and short of it - polyphosphate, PPK and bacterial survival.
Brown et al. (2008)
2 more

2 mentions without citation

Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules.
Lorenzo-Orts et al. (2019)
1 more