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› 2qbl

X-ray diffraction
1.8Å resolution

Crystal structure of ferric G248T cytochrome P450cam

Released:
DOI: 10.2210/pdb2qbl/pdb
Model geometry
Fit model/data
PDB entry 2qbl coloured by chain, front view.
PDB entry 2qbl coloured by chain, side view.
PDB entry 2qbl coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Alteration of P450 distal pocket solvent leads to impaired proton delivery and changes in heme geometry.
Makris TM, von Koenig K, Schlichting I, Sligar SG
Biochemistry 46 14129-40 (2007)
PMID: 18001135

Function and Biology Details

Reaction catalysed: 
[2 reduced [2Fe-2S]-[putidaredoxin] + (1R,4R)-camphor + O2 + 2 H(+) =(1R,4R,5R)-5-hydroxycamphor + 2 oxidized [2Fe-2S]-[putidaredoxin] + H2O.]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 421 amino acids
Theoretical weight: 47.59 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00183 (Residues: 1-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand K 1 x K
Ligand CAM 1 x CAM
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 64.09Å b: 64.59Å c: 106.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.264
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Heme enzyme structure and function.
Poulos TL. (2014)
6 more

1 mention without citation

Conformational plasticity and structure/function relationships in cytochromes P450.
Pochapsky et al. (2010)