› 2qcu

X-ray diffraction
1.75Å resolution

Crystal structure of Glycerol-3-phosphate Dehydrogenase from Escherichia coli

Released:
DOI: 10.2210/pdb2qcu/pdb
Model geometry
Fit model/data
PDB entry 2qcu coloured by chain, front view.
PDB entry 2qcu coloured by chain, side view.
PDB entry 2qcu coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of glycerol-3-phosphate dehydrogenase, an essential monotopic membrane enzyme involved in respiration and metabolism.
Yeh JI, Chinte U, Du S
Proc Natl Acad Sci U S A 105 3280-5 (2008)
PMID: 18296637

Function and Biology Details

Reaction catalysed: 
sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aerobic glycerol-3-phosphate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 501 amino acids
Theoretical weight: 56.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P13035 (Residues: 1-501; Coverage: 100%)
Gene names: JW3389, b3426, glpD, glyD
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
6 bound ligands:
Ligand BOG 6 x BOG
Ligand SO4 4 x SO4
Ligand PO4 2 x PO4
Ligand TAM 3 x TAM
Ligand EDO 30 x EDO
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X10SA, APS BEAMLINE 22-ID
Spacegroup: I222
Unit cell:
a: 113.791Å b: 114.097Å c: 192.801Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.24
Expression system: Escherichia coli

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Citations

10 review citations

The sites and topology of mitochondrial superoxide production.
Brand MD. (2010)
9 more

14 mentions without citation

Monotopic Membrane Proteins Join the Fold.
Allen et al. (2019)
13 more