› 2qee

X-ray diffraction
1.65Å resolution

Crystal structure of putative amidohydrolase BH0493 from Bacillus halodurans C-125

Released:
DOI: 10.2210/pdb2qee/pdb
PDB entry 2qee coloured by chain, front view.
PDB entry 2qee coloured by chain, side view.
PDB entry 2qee coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Entry authors: Malashkevich VN, Toro R, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BH0493 protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 437 amino acids
Theoretical weight: 51.22 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KFI6 (Residues: 2-427; Coverage: 100%)
Gene name: BH0493
Structure domains: uronate isomerase, domain 2, chain A

Ligands and Environments

3 bound ligands:
Ligand CL 16 x CL
Ligand MG 4 x MG
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P1
Unit cell:
a: 121.995Å b: 122.208Å c: 127.582Å
α: 78.85° β: 78.95° γ: 80.92°
R-values:
R R work R free
0.189 0.187 0.224
Expression system: Escherichia coli BL21(DE3)

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