› 2qfj

X-ray diffraction
2.1Å resolution

Crystal Structure of First Two RRM Domains of FIR Bound to ssDNA from a Portion of FUSE

Released:
DOI: 10.2210/pdb2qfj/pdb
PDB entry 2qfj coloured by chain, front view.
PDB entry 2qfj coloured by chain, side view.
PDB entry 2qfj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dimerization of FIR upon FUSE DNA binding suggests a mechanism of c-myc inhibition.
Crichlow GV, Zhou H, Hsiao HH, Frederick KB, Debrosse M, Yang Y, Folta-Stogniew EJ, Chung HJ, Fan C, De la Cruz EM, Levens D, Lolis E, Braddock D
EMBO J 27 277-89 (2008)
PMID: 18059478

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115800 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Poly(U)-binding-splicing factor PUF60 Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 23.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UHX1 (Residues: 118-316; Coverage: 36%)
Gene names: FIR, PUF60, ROBPI, SIAHBP1
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
DNA (5'-D(*DTP*DCP*DGP*DGP*DGP*DAP*DTP*DTP*DTP*DTP*DTP*DTP*DAP*DTP*DTP*DTP*DTP*DGP*DTP*DGP*DTP*DTP*DAP*DTP*DT)-3') Chain: C
Molecule details ›
Chain: C
Length: 25 nucleotides
Theoretical weight: 7.7 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P31
Unit cell:
a: 63.135Å b: 63.135Å c: 82.589Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.258 0.258 0.295
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Single-stranded DNA-binding proteins: multiple domains for multiple functions.
Dickey et al. (2013)
5 more

11 mentions without citation

INVOLVED IN DE NOVO 2-containing complex involved in RNA-directed DNA methylation in Arabidopsis.
Ausin et al. (2012)
10 more