› 2qfl

X-ray diffraction
1.9Å resolution

Structure of SuhB: Inositol monophosphatase and extragenic suppressor from E. coli

Released:
DOI: 10.2210/pdb2qfl/pdb
PDB entry 2qfl coloured by chain, front view.
PDB entry 2qfl coloured by chain, side view.
PDB entry 2qfl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of the R184A mutant of the inositol monophosphatase encoded by suhB and implications for its functional interactions in Escherichia coli.
Wang Y, Stieglitz KA, Bubunenko M, Court DL, Stec B, Roberts MF
J Biol Chem 282 26989-26996 (2007)
PMID: 17652087

Function and Biology Details

Reaction catalysed: 
Myo-inositol phosphate + H(2)O = myo-inositol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nus factor SuhB Chain: A
Molecule details ›
Chain: A
Length: 267 amino acids
Theoretical weight: 29.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0ADG4 (Residues: 1-267; Coverage: 100%)
Gene names: JW2517, b2533, ssyA, suhB
Sequence domains: Inositol monophosphatase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand EEE 1 x EEE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ DW
Spacegroup: C2
Unit cell:
a: 89.767Å b: 45.447Å c: 71.758Å
α: 90° β: 125.43° γ: 90°
R-values:
R R work R free
0.213 0.209 0.286
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Regulation of bacterial gene expression by ribosome stalling and rescuing.
Jin et al. (2016)
10 other articles

4 mentions without citation

Dimeric interactions and complex formation using direct coevolutionary couplings.
dos Santos et al. (2015)
3 more