› 2qg3

X-ray diffraction
1.95Å resolution

CRYSTAL STRUCTURE OF A TYW3 METHYLTRANSFERASE-LIKE PROTEIN (AF_2059) FROM ARCHAEOGLOBUS FULGIDUS DSM 4304 AT 1.95 A RESOLUTION

Released:
DOI: 10.2210/pdb2qg3/pdb
PDB entry 2qg3 coloured by chain, front view.
PDB entry 2qg3 coloured by chain, side view.
PDB entry 2qg3 coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 7-((3S)-(3-amino-3-carboxypropyl))-4-demethylwyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-(3-amino-3-carboxypropyl))wyosine(37) in tRNA(Phe)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 23.8 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O28220 (Residues: 1-196; Coverage: 100%)
Gene names: AF_2059, taw3
Sequence domains: Methyltransferase TYW3
Structure domains: tRNA wybutosine-synthesizing-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2, SSRL BEAMLINE BL11-1
Spacegroup: P42
Unit cell:
a: 59.334Å b: 59.334Å c: 109.102Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.253
Expression system: Escherichia coli

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