› 2qgv

X-ray diffraction
2.7Å resolution

Crystal structure of hydrogenase-1 operon protein hyaE from Shigella flexneri. Northeast Structural Genomics Consortium Target SfR170

Released:
DOI: 10.2210/pdb2qgv/pdb
PDB entry 2qgv coloured by chain, front view.
PDB entry 2qgv coloured by chain, side view.
PDB entry 2qgv coloured by chain, top view.
Source organism: Shigella flexneri 2a str. 301
Entry authors: Forouhar F, Su M, Seetharaman J, Benach J, Wang D, Fang Y, Cunningham K, Ma L-C, Xiao R, Liu J, Baran MC, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-104470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydrogenase-1 operon protein hyaE Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 140 amino acids
Theoretical weight: 16.11 KDa
Source organism: Shigella flexneri 2a str. 301
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2S4MV65 (Residues: 1-132; Coverage: 100%)
Gene names: SF0977, hyaE
Sequence domains: Hydrogenase-1 expression protein HyaE
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 55.172Å b: 215.777Å c: 64.069Å
α: 90° β: 94.8° γ: 90°
R-values:
R R work R free
0.256 0.253 0.292
Expression system: Escherichia coli

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