› 2qgz

X-ray diffraction
2.4Å resolution

Crystal structure of a putative primosome component from Streptococcus pyogenes serotype M3. Northeast Structural Genomics target DR58

Released:
DOI: 10.2210/pdb2qgz/pdb
PDB entry 2qgz coloured by chain, front view.
PDB entry 2qgz coloured by chain, side view.
PDB entry 2qgz coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M3
Entry authors: Seetharaman J, Chen Y, Wang D, Fang Y, Cunningham K, Ma L-C, Xia R, Liu J, Baran MC, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative primosome component Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 35.26 KDa
Source organism: Streptococcus pyogenes serotype M3
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2UTE5 (Residues: 1-300; Coverage: 100%)
Gene names: SpyM3_0248, dnaI
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P41212
Unit cell:
a: 76.169Å b: 76.169Å c: 119.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.254
Expression system: Escherichia coli

Quick links